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在呼吸机引起的肺损伤后,机械应变和巨相关的肺炎的区域分布:一项实验研究

Francesco Liggieri^1,2, Elena Chiodaroli^1,3, Mariangela Pellegrini^1,4

¹The Hedenstierna Laboratory, Department of Surgical Sciences, Uppsala University, Akademiska Sjukhuset-Ing. 40, Tr. 3, 75185, Uppsala, Sweden.

Intensive care medicine experimental

|September 3, 2024

概括

肺部的机械应变与呼吸机诱导的肺损伤 (VILI) 中激活的巨细胞分布相关. 这项研究使用[Ga]Ga-DOTA-TATE PET成像绘制巨细胞激活的地图,揭示了肺部应变和炎症之间的联系.

科学领域:

肺部医学肺部医学
放射学放射学是一门学科.
炎症研究炎症研究

背景情况:

对M1表型的膜巨细胞激活是呼吸器诱导肺损伤 (VILI) 病理生理学的关键.
增加的肺张力是VILI决定因素,但其与巨细胞激活的直接联系尚不清楚.
[⁶⁸Ga]Ga-DOTA-TATE PET成像的向体静止素受体亚型2 (SSTR2),通过激活的巨细胞过度表达.

研究的目的:

在猪VILI模型中调查区域肺菌株和激活巨细胞分布之间的地形相关性.
评估[⁶⁸Ga]Ga-DOTA-TATE的吸收是否反映了VILI中的机械应变模式.
量化肺部应变和巨细胞激活之间的关系在voxel水平.

主要方法:

七只猪接受了VILI,三只作为对照.
肺部CT扫描和机械记录在增加的潮体积.
获得了Ga-DOTA-TATE PET扫描,定制脚本生成了应变和吸收地图.
线性相互信息 (LMI) 量化了与Voxel相关的正常菌株 (VoStrain) 和Ga-DOTA-TATE吸收之间的相关性.

主要成果:

维利猪的VoStrain和标准化摄入值比率 (SUVR) 显著高于对照对象,在半腹膜和胸部中部水平.
无论是VoStrain还是SUVR都沿着引力轴增加,在依赖肺部区域达到峰值.
与对照组相比,VILI猪的LMI显著更高,这表明相关性更强.

结论:

在猪VILI模型中,肺压力和[⁶⁸Ga]Ga-DOTA-TATE在声素水平的吸收之间存在地形相关性.
机械变化和炎症细胞激活在VILI中密切相关.
这项研究提供了第一个使用多模式成像技术对这种关系的voxel-by-voxel检查.

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