相关实验视频

Updated: Jun 14, 2025

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

线粒体综合体I子单元NDUFS2的降低对于猪在排卵后衰老期间的卵细胞质量至关重要

Dongjie Zhou^1,2, Song-Hee Lee¹, Xiao-Han Li¹

¹Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|September 3, 2024

概括

尼古丁胺胺氨基二核酸 (NADH) 脱酶[乌比金]铁硫蛋白2 (NDUFS2) 对于在排卵后衰老 (POA) 期间保持猪卵细胞质量至关重要. 减少NDUFS2会影响卵细胞形态和随后的胚胎发育.

科学领域:

生殖生物学生殖生物学
线粒体生物学线粒体生物学
细胞衰老细胞衰老

背景情况:

尼古丁胺胺氨基二核酸 (NADH) 脱酶[乌比金]铁硫蛋白2 (NDUFS2) 水平在老化组织中下降,可能与与年龄相关的疾病有关.
衰老会导致线粒体缺陷,包括生物生成中断,功能障碍,膜潜能变化和活性氧物种增加,这可能与NDUFS2表达有关.

研究的目的:

调查NDUFS2在猪卵子排卵后衰老 (POA) 中的作用.
检查NDUFS2淘汰对POA期间线粒体功能和卵细胞质量的影响.

主要方法:

在猪卵细胞排卵后衰老期间评估NDUFS2mRNA表达变化.
使用dsRNA微注射在MII阶段执行NDUFS2敲击 (KD).
评估卵细胞形态,芽细胞发育和关键线粒体蛋白质水平 (PGC1α,GSNOR) 在NDUFS2后的KD和衰老.

主要成果:

在POA48小时后,NDUFS2mRNA表达显著下降.
在NDUFS2 Knockdown中显著损害了卵细胞形态和随后的胚胎细胞发育.
在48小时的衰老后,NDUFS2 KD导致PGC1α水平降低,GSNOR水平降低.

结论:

在猪的排卵后衰老过程中,NDUFS2在保持卵细胞质量方面发挥着至关重要的作用.
在卵细胞衰老过程中,NDUFS2对于线粒体生物发生和维持细胞氧化还原平衡至关重要.

关键词:

在 NADH 脱水酶.在NDUFS2中,线粒体中的线粒体.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪排卵后的衰老排卵后衰老

更多相关视频

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

相关概念视频

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Meiosis II

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

Meiosis vs. Mitosis

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...