发病率和特征ESBL产生的沙门氏菌在中国的韦邦,中国
Xuesong Wang1, Binglei Wang2, Xiaohong Lu1
11Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China.
Acta microbiologica et immunologica Hungarica
|September 3, 2024
概括
这项研究在韦邦一家医院发现了CTX-M产生沙门氏菌,揭示了对常见抗生素如安培和 ceftriaxone的高耐药性. 主导的扩展谱β-乳糖酶 (ESBL) 基因型是CTX-M-55,通常与ISEcp1插入序列相关.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 传染性疾病 传染性疾病
背景情况:
- 扩展谱β-乳糖酶 (ESBL) 生产细菌,特别是沙门氏菌种,在医疗保健环境中构成重大威胁.
- 了解沙门氏菌抗生素耐药性的流行和遗传基础对于有效的治疗和感染控制至关重要.
研究的目的:
- 为了调查在韦邦医院的blaCTX-M阳性沙门氏菌种的流行率和抗生素耐药性模式.
- 描述这些隔离品中的blaCTX-M基因的遗传特征,包括血清型,序列类型和遗传环境.
主要方法:
- 从住院患者中分离和识别沙门氏菌株 (2018年1月-2023年4月).
- 全基因组测序 (Illumina),CTX-M基因鉴定 (CARD),抗生素敏感性测试 (BD PhoenixTM M50),MLST和血型鉴定 (SeqSero2) 以及遗传环境分析 (Isfinder,BLASTn).
主要成果:
- 检测到34种产生CTX-M的沙门氏菌菌株. 最常见的血清型是沙门氏菌1,4,[5],12:i:- (ST34),其次是沙门氏菌 (ST11).
- 对安培素 (97.06%), ceftriaxone (94.12%) 和 ceftazidime (58.83%),观察到高耐药率.
- 主要的blaCTX-M基因是blaCTX-M-55 (47.06%),其次是 blaCTX-M-14,也是最常见的基因. 另外还发现了40个抗生素耐药性基因,包括mcr-1.1.1. 插入序列ISEcp1经常被发现在blaCTX-M基因的上游.
结论:
- 沙门氏菌spp. 的临床分离物. 在这个医院携带多个抗生素耐药性基因.
- 主导的ESBL基因型是CTX-M-55,它与插入序列ISEcp1相关,突出了其传播的关键机制.
- 这些发现强调了对抗多药耐药沙门氏菌的持续监测和有针对性的干预措施的必要性.
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