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内皮化支气管膜肺器官模拟内皮细胞对受伤的反应

Anna-Lena Ament^1,2, Monika Heiner^1,3, Marie Christin Hessler¹

¹Department of Medicine V (Internal Medicine, Infectious Diseases and Infection Control), Universities of Giessen and Marburg Lung Center (UGMLC), Giessen, Germany.

American journal of respiratory cell and molecular biology

|September 3, 2024

概括

研究人员通过将内皮细胞 (EC) 添加到支气管膜肺器官 (BALO) 中,开发出了一种新的肺器官模型. 这种增强的模型允许研究肺内皮对损伤的反应ex vivo.

科学领域:

* 发育生物学发育生物学
*再生医学是一种再生医学.
* 呼吸系统研究

背景情况:

*有机体模型对于研究发育和疾病非常有价值.
* 现有的肺类器官缺乏集成的内皮细胞区.
* 需要更复杂的小鼠肺器官系统.

研究的目的:

* 开发一种新型的小鼠肺器官模型,其中包含一个内皮.
* 增强现有的支气管膜肺有机体 (BALO) 模型的细胞和结构复杂性.
* 创建一个研究肺内皮对损伤的反应的平台ex vivo.

主要方法:

*微注射FACS排序的肺内皮细胞 (ECs) 进入分化的BALO培养物.
*使用标记物 (CD31,血管内皮卡德林) 和管形成试验来表征EC.
*共聚焦和电子显微镜以评估EC集成和与膜上皮细胞的相互作用.

主要成果:

*注射的ECs集成到BALO膜结构中,与膜上皮细胞对齐.
*检测到的EC标志物 (Car4,Aplnr) 表明了微血管表型.
*内皮化BALOs通过释放细胞因子和上调ICAM-1来应对损伤 (LPS,流感A病毒).

结论:

*成功创建了一种新的内皮化肺器官模型 (BALO).
*与以前的版本相比,这个模型表现出更高的细胞和结构复杂性.
* 该模型作为一个平台,用于研究肺内皮对外伤的肺内皮反应.

关键词:

支气管气管肺的有机体内皮细胞生物学内皮细胞生物学肺炎肺炎肺炎肺炎

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