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相关概念视频

RNA Splicing01:32

RNA Splicing

56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.2K
RNA Stability01:53

RNA Stability

33.4K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.4K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.6K
pre-mRNA Processing02:01

pre-mRNA Processing

52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K

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相关实验视频

Updated: Jun 14, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

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mRNA末端之间的分离比预期的更可变.

Nancy Gerling1, J Alfredo Mendez2, Eduardo Gomez3

  • 1Institute of Physics, Biological Physics Laboratory, San Luis Potosi, Mexico.

FEBS open bio
|September 3, 2024
PubMed
概括

传递 RNA (mRNA) 的端到端分离在整个生物体中得到保护,影响翻译启动和表型稳定性. 较短的分离可能会增强表型的稳定性,而变性则表明了调节机制.

关键词:
在RNA外部循环中.RNA的二级结构是RNA的二级结构.轮长度 轮长度 轮长度它们是mRNARNA.现型稳定性 现型稳定性

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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相关实验视频

Last Updated: Jun 14, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物物理学的生物物理.

背景情况:

  • 有效的信使RNA (mRNA) 循环化对于高效的翻译启动至关重要.
  • 以前的研究表明mRNA末端之间存在很小的内在分离,这表明了循环化的分子内安排.
  • 这种近距离的生物学含义在很大程度上是未知的.

研究的目的:

  • 调查mRNA末端近距离是否是各种生物体中保存的特征.
  • 探索mRNA端到端分离的功能后果.
  • 了解mRNA二次结构在翻译中的作用.

主要方法:

  • 对mRNA二次结构的计算分析.
  • 研究了来自17种不同生物体的274个完全本地mRNA分子.
  • 计算外部环轮长度 (CL) 作为端到端分离的指数.

主要成果:

  • 观察到mRNA端到端分离的显著变化 (0.5931.8nm),超过之前的报告和随机序列.
  • 确定大于18.5nm的分离是不受欢迎的.
  • 短暂的分离与表型稳定性相关.

结论:

  • 一个生物学机制可能驱动了mRNA端到端分离的观察到的变异性.
  • mRNA外环轮长度 (CL) 对翻译启动有潜在的相关性.
  • 短的mRNA端到端分离可能有助于表型稳定.