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Updated: Jun 14, 2025

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开发用于膜蛋白降解的因特林辅助双特异性阿帕特马基默体

Weidi Sun1,2, Hui Zhang2,3, Wanlin Xie2

  • 1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Journal of the American Chemical Society
|September 3, 2024
PubMed
概括

lysosome-targeting chimeras (LYTACs) 提供了一种降解膜蛋白的新方法. 研究人员开发了因整蛋白α3β1 (ITGA3B1) 促成的双特异性体仿真体 (ITGBACs),用于改善蛋白质降解和癌症治疗.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Tail-anchoring of Proteins in the ER Membrane

Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
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