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Updated: Jun 14, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

一个用户友好的CRISPR/Cas9系统用于Neurospora crassa的突变发生

Stefanie Grüttner¹, Frank Kempken²

¹Abteilung Botanische Genetik und Molekularbiologie, Botanisches Institut und Botanischer Garten, Christian-Albrechts-Universität zu Kiel, Olshausenstraße 40, 24098, Kiel, Germany. sgruettner@bot.uni-kiel.de.

Scientific reports

|September 3, 2024

概括

这项研究引入了一个用户友好的CRISPR/Cas9系统用于Neurospora crassa,使得快速的基因操纵. 这种高效的系统加速了这种重要的真核模型生物体中功能丧失突变的产生.

科学领域:

分子生物学分子生物学
菌类学菌类学是指菌类学.
遗传学遗传学是一个

背景情况:

神经体 (Neurospora crassa) 是一种有价值的真核模型生物体,用于遗传学研究.
在N. crassa的传统基因操纵是耗时和劳动密集的.

研究的目的:

为N. crassa.开发一个高效和用户友好的CRISPR/Cas9系统.
为了简化生成功能丧失突变体的过程.

主要方法:

开发了一个CRISPR/Cas9系统,在N. crassa基因组中集成了cas9序列.
通过电穿孔引入裸体导向RNA.
使用耐循环素-1 (csr-1) 作为可选择的标记物和向的N-乙乙胺胺基酶-2 (naa-2) 进行编辑.

主要成果:

实现了100%的编辑效率,用于选择中的可选择标记器csr-1.
成功编辑了不可选择的 naa-2 基因,证明了系统的多功能性.
同时准csr-1和naa-2增加了识别非选择性突变的可能性.

结论:

开发的CRISPR/Cas9系统为N. crassa变异的传统方法提供了快速有效的替代方案.
该系统简化了基因操纵,加速了对这种模型生物的研究.

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Published on: June 9, 2020

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

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