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探索氨酸-氨酸丰富的拼接因子:口腔癌失调的潜在预测标志物

Sakshi Sharma¹, Manasi Mittal¹, Akanksha Shukla¹

¹Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi, 110007, India.

|September 3, 2024

概括

失调的拼接因子,富含氨酸和氨酸的拼接因子 (SRSFs),在口腔癌中被上调. 这项研究研究了口腔癌细胞系和患者样本中的SRSF家族表达,揭示了它们在癌症进展中的关键作用.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
遗传学是一个遗传学.

背景情况:

不调节的拼接在癌症中很常见.
富含氨酸和氨酸的拼接因子 (SRSFs) 调节基因表达.
许多SRSF家族成员在癌症中的作用尚未得到充分研究.

研究的目的:

在口腔癌中研究整个SRSF家族 (SRSF1-SRSF12) 的表达特征.
确定SRSF家族表达和口腔癌进展之间的关联.
为了确定口腔癌的潜在SRSF生物标志物.

主要方法:

在23个口腔癌细胞系上进行定量实时PCR (qRT-PCR).
在口腔癌患者样本 (癌症前期,早期,晚期) 和相邻的非瘤组织中分析SRSF家族转录水平.
使用来自癌症基因组图谱 (TCGA) 的转录组学数据对口腔状细胞癌 (OSCC) 的验证.

主要成果:

在所有测试的口腔癌细胞系中,SRSF3,SRSF9和SRSF10都被上调.
在晚期口腔癌患者中,SRSF1,SRSF3,SRSF7,SRSF9,SRSF10和SRSF11的调节显著上升.
在OSCC患者中,TCGA数据证实了SRSF1,SRSF4,SRSF9,SRSF10和SRSF11的上调.

结论:

SRSF家族成员在口腔癌中发挥了关键作用.
特定的SRSFs的升级与口腔癌的进展相关.
SRSFs代表了改变拼接动态的潜在关键参与者,并可能作为口腔癌中的生物标志物.

关键词:

另一个替代拼接.癌症的进展情况.细胞系细胞系细胞系失调的表达方式失调.口腔状细胞癌的癌症.有关RNA拼接因素的RNA拼接因素在SRSF中,您可以使用SRSF.这是一个TCGA数据库.

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