相关实验视频

Updated: Jun 14, 2025

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

瘤微环境响应的基于的纳米调节器激活cGAS-STING通路,以增强先天免疫系统反应

Xiayi Liang¹, Duo Wang², Yuanquan Zhao³

¹Department of Hepatobiliary Surgery, Department of Medical Ultrasound, Tumor Hospital of Guangxi Medical University, Guangxi Medical University, No. 71 Hedi Road, Nanning, 530021, Guangxi, China.

Journal of nanobiotechnology

|September 3, 2024

概括

一种新型的基于的纳米调节器加载了泽布拉林,并修改了酸,通过破坏瘤细胞和激活循环GMP-AMP干扰素基因合成酶刺激 (cGAS-STING) 途径来增强癌症治疗,从而促进免疫反应.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症研究癌症研究

背景情况:

离子 (Mn2+) 和瘤 lysing 辅助剂通过循环 GMP-AMP 干扰素基因合成酶刺激器 (cGAS-STING) 途径创建纳米调节器,以提高癌症治疗的疗效.
开发诱导DNA损伤和增强cGAS-STING活动的纳米调节器对于未来的癌症研究至关重要.

研究的目的:

合成和描述一种新的抗瘤纳米调节器,用于增强癌症免疫治疗.
研究纳米调节器诱导瘤细胞死亡和激活cGAS-STING通路的能力.

主要方法:

通过加载二氧化纳米片 (M-NS) 与泽布拉林 (Zeb) 并用氨酸 (HA) 修改表面,合成了一个纳米调节器.
评估了纳米调节器在瘤微环境中的分散性,生物安全性和治疗效果.
评估了诱导DNA损伤,活性氧物种 (ROS) 生产和cGAS-STING通路激活.

主要成果:

合成的纳米调节器 (Zeb@MH-NS) 显示出良好的分散性和生物安全性.
在瘤微环境中释放的Mn2+和Zeb诱导了瘤细胞DNA/线粒体损伤和编程细胞死亡.
观察到增强的双链DNA (dsDNA) 积累,强大的cGAS-STING通路激活,增加I型干扰素的产生,并增加了促炎性细胞因子分泌.
纳米调节器促进了树突细胞的成熟,细胞毒性T淋巴细胞的透和自然杀手细胞的招募.

结论:

基于的混合纳米调节剂,通过刺激先天免疫力来增强抗瘤疗法.
这种方法为癌症免疫治疗和潜在的临床转化提供了有希望的新方向.

关键词:

这是天生的免疫力.的免疫疗法的免疫疗法瘤微环境是一个微环境.在cGAS-STING路径中.

更多相关视频

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...