用LC-QTOF-MS识别以前未知的化合物的逐步程序,以甲基类同类SL-164中毒为例.
Helena Fels1, Simon Franz1, Torsten Dame1
1Forensic Toxicological Centre (FTC), Munich, Germany.
Drug testing and analysis
|September 4, 2024
概括
一种新型设计药物SL-164及其代谢物在疑似过量病例中使用液体染色学四极点飞行时间质谱法 (LC-QTOF-MS) 确定. 这突显了LC-QTOF-MS在生物样本中检测未知物质方面的能力.
科学领域:
- * 法医毒理学 * 法医毒理学
- * 分析化学 分析化学
- * 药理学 药理学 药理学
背景情况:
- * 一名22岁的男性呈现出改变精神状态,怀疑药物过量.
- *对血清和尿液样本进行了例行毒理学分析.
- *初步分析检测到吗啡和巴比他.
研究的目的:
- * 在疑似药物过量后的生物样本中识别未知物质.
- * 调查设计药物和新型精神活性物质的存在.
- * 为了确认身份和量化新发现的甲基类比物,SL-164.
主要方法:
- * 液体染色学-四极-飞行时间质谱学 (LC-QTOF-MS) 用于初始查和识别.
- *核磁共振 (NMR) 光谱用于SL-164.4的结构确认和纯度评估.
- * 血清中的SL-164及其尿液中的代谢物的定量分析.
主要成果:
- *发现了吗啡和巴比他.
- *检测到两种未知的化合物,最初被怀疑是设计者二,被检测到.
- *SL-164,一种甲基类比物,及其氧代谢物暂时被确定.
- *SL-164在血清中被证实并定量为390ng/mL.
- * 在尿液中检测到SL-164的三种单氧化代谢物.
结论:
- *LC-QTOF-MS是一种强大的工具,用于识别生物矩阵中的未知化合物.
- *SL-164是一种新型设计药物,可以在临床毒理学中检测和量化.
- *这项研究表明,新兴的精神活性物质在药物市场上出现了.
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