TLR3和TLR4激活对MSC介导的免疫调节的不同影响
Urvashi Kaundal1,2, Aruna Rakha2
1Department of Translational and Regenerative Medicine, Postgraduate Institute of Medical Education and Research, Sector-12, Chandigarh, 160012, India.
Biochemistry and biophysics reports
|September 4, 2024
概括
收费类受体 (TLR) 激活许可介酶体 stromal 细胞 (MSCs) 用于免疫调节. 用TLR3为原料的MSC增强了调节性T细胞并抑制了T细胞的增殖,这表明了治疗应用的潜力.
科学领域:
- 免疫学和再生医学 免疫学和再生医学
- 细胞和分子生物学 细胞和分子生物学
背景情况:
- 介酶体 stromal 细胞 (MSCs) 显示出治疗前景,但在体内表现出不同的患者反应.
- 主体微环境,特别是收费类受体 (TLR),影响MSC功能.
- 对于TLR激活对MSC介导免疫调节的影响尚不清楚.
研究的目的:
- 调查TLR3和TLR4激活在授权MSCs免疫调节中的作用.
- 确定TLR激活的MSC如何影响免疫细胞群和功能.
主要方法:
- MSCs被TLR3和TLR4激动剂激活.
- 分析了激活的MSCs的细胞因子和化学因子表达特征.
- 在体外共同培养模型中评估了TLR激活的MSCs对T细胞子集 (天真,效应,记忆,调控) 和B细胞的影响.
主要成果:
- TLR3的激活增加了G-CSF和IL-10的调节,而TLR4的激活增加了CXCL-1,CXCL-10和CXCL-12.
- TLR3-MSCs增加了天真T细胞和减少了效应/记忆T细胞,对增加调节性T细胞的趋势不显著.
- TLR3-MSCs抑制T细胞增殖,并显示调控性B细胞的非显著增加;TLR4-MSCs对T细胞概况没有显著影响.
结论:
- MSCs的TLR3化调节它们的免疫调节功能,特别是影响T细胞种群和增殖.
- 了解TLR许可对于优化MSC治疗和克服体内可变反应至关重要.
相关概念视频
01:24T Cell Types and Functions
963
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
963
01:19Mesenchymal Stem Cells
4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
01:22T Cell Activation and Clonal Selection
691
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
691