一个IL-17-DUOX2轴控制着Candida albicans的胃肠道殖民活动
bioRxiv : the preprint server for biology
|September 4, 2024
概括
肠道中对Candida albicans的宿主反应涉及Duox2和IL-17信号传递. 这项研究揭示了Duox2的活性因真菌殖民而令人惊地减少,影响了宿主-真菌相互作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 菌 (Candida albicans) 是一种常见的肠道真菌,引起机会性感染.
- 调节C. albicans肠道殖民的宿主-病原体相互作用尚不清楚.
研究的目的:
- 为了研究宿主基因表达,以应对C. albicans在小鼠肠道中的殖民.
- 定义Duox2/Duoxa2和IL-17信号传递在C. albicans肠道殖民中的作用.
主要方法:
- 在被C. albicans殖民的无细菌小鼠中,对大肠和大肠组织的表达式概况.
- 利用缺乏IL-17受体和DUOX2活性的小鼠来评估宿主反应和真菌殖民.
- 使用结肠状体模型研究IL-17A诱导的基因表达.
主要成果:
- 杜奥克斯2和杜奥克萨2被C. albicans上调,需要酵母 - 基因过渡和候选解.
- 对于Duox2/Duoxa2诱导来说,IL-17受体信号传递是必要的.
- 丢失DUOX2功能减少了C. albicans在以后的时间点上的殖民,并增加了形形式.
- 在DUOX2缺乏的小鼠中,IL-17A水平升高,这表明交叉调节.
结论:
- 一个新的宿主IL-17-DUOX2轴参与调节C. albicans肠道殖民.
- C. albicans的细丝和殖民是由真菌因素和宿主DUOX2活动之间的相互作用来调节的.
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