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Updated: Jun 14, 2025

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
11:49

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Published on: July 28, 2014

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在具有敏感构造生物传感器的细胞中直接检测内源性Gαi活性

Alex Luebbers1, Remi Janicot1, Jingyi Zhao1

  • 1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA.

bioRxiv : the preprint server for biology
|September 4, 2024
PubMed
概括

这项研究引入了一种新的光学生物传感器,可通过G蛋白结合受体 (GPCRs) 稳定检测内源G蛋白激活. 生物传感器克服了现有方法的局限性,使得本地信号通路的敏感和无干扰分析成为可能.

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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