在Mycobacterium tuberculosis中检测到的细菌RNA转录的描述 - 使用单细胞RNA测序测序从外围人体颗粒瘤中感染的细胞
Philip J Moos1, Allison F Carey2, Jacklyn Joseph3
1Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84112 USA.
bioRxiv : the preprint server for biology
|September 4, 2024
概括
研究人员在人类单细胞RNA测序库中检测到Mycobacterium tuberculosis (Mtb) 转录. 这些细菌序列显示出显著的变异,表明了对宿主防御或治疗的潜在Mtb适应机制.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 结核菌菌 (Mtb) 是一个主要的全球健康威胁,导致严重的发病率和死亡率.
- 在真核细胞测序研究中,细菌转录通常被忽视为噪音.
- 以前的研究暗示了Mtb RNA中的多基化转录.
研究的目的:
- 为了研究在真核细胞mRNA测序库中捕获的Mtb转录的存在和特征.
- 从临床样本中探索Mtb转录中的序列变异.
- 假设这些变异对Mtb适应的潜在影响.
主要方法:
- 使用了10X Genomics单细胞RNA测序 (scRNA-seq) 与多T原始体.
- 包括H37Rv Mtb参考基因组用于读取对齐.
- 分析的测序来自巴布亚新几内亚结核病患者的细针吸血样本.
主要成果:
- 在细胞培养和患者样本中始终检测到Mtb核糖体RNA (rrs, rrl) 和其他基因转录.
- 解释的mtb读取与宿主细胞UMI相关,表明宿主细胞感染.
- 在多名患者中,与H37Rv参考基因组相比,在Mtb rrs/rrl读数中观察到显著的序列变异 (>90%).
结论:
- Mtb转录可以无意中被捕获和分析在真核细胞scRNA-seq.
- 在Mtb转录中检测到的序列变化是非随机的,可能表明适应机制.
- 这些变异可能代表了Mtb的新策略,以提高对宿主防御或化疗的适应性.
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