亚当与大型蛋白质复合体相互作用,以调节 histone 修饰,基因表达和拼接.
bioRxiv : the preprint server for biology
|September 4, 2024
概括
亚当13是一种金属蛋白酶,通过影响基因组修饰来调节神经 (CNC) 细胞基因表达. 这对于面部发育和CNC细胞迁移至关重要.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 神经 (CNC) 细胞是脊椎动物面部发育的重要干细胞.
- 在CNC细胞的诱导和迁移过程中,ADAM (A 解体和金属蛋白酶) 蛋白质至关重要.
- 之前的工作确立了Adam13与转录因子Arid3a的关联,用于基因调节.
研究的目的:
- 为了研究Adam13在CNC细胞内调节质子修饰中的作用.
- 阐明Adam13影响Arid3a结合和基因表达的机制.
- 了解Adam13对RNA拼接的影响及其对CNC迁移的影响.
主要方法:
- 在CNC细胞中分析基质子修饰的分析.
- 调查Arid3a与tfap2α促进体结合的存在和缺席的Adam13.
- 对基因表达的评估,重点是CNC迁移关键基因和tfap2α变异.
- 同免疫沉试验用于识别与Adam13和ADAM9相关的蛋白质,这些蛋白质参与了基因素修饰和RNA剪接.
主要成果:
- 发现Adam13可以调节CNC细胞中的组素修饰.
- 结合tfap2α促进体的arid3a需要Adam13,促进一个CNC特定的tfap2α变体.
- 这种tfap2α变体激活了CNC迁移所必需的基因.
- 亚当13和ADAM9与参与基质子修饰和RNA剪接的蛋白质结合,这种过程在亚当13丢失时受损.
结论:
- 亚当13通过基因素修饰在CNC细胞中调节基因表达方面发挥着重要作用.
- 亚当13-Arid3a相互作用对tfap2α表达至关重要,推动了CNC迁移.
- 亚当蛋白可能作为细胞外传感器起作用,影响染色质可访问性和基因/RNA拼接调节.
相关概念视频
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Histone Modification
13.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.2K
Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K
What is Gene Expression?
8.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.5K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K
Regulation of Expression at Multiple Steps
875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875


