通过皮肤局部环境中的细胞抑制黑色素瘤的进展
Yilun Ma1,2,3, Mohita Tagore2, Miranda V Hunter2
1Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, NY, USA.
bioRxiv : the preprint server for biology
|September 4, 2024
概括
黑色素瘤附近的角质细胞可以经历一次上皮层-介质细胞过渡 (EMT). 在质细胞中引发的扭曲EMT通过改变瘤微环境 (TME) 内的细胞相互作用,令人惊地减少了黑色素瘤的入侵和改善了生存率.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 角质细胞是黑色素瘤微环境中的关键细胞.
- 黑色素瘤的进展受到状细胞行为的影响.
- 角质细胞在与黑色素瘤相互作用时,可以经历类似于上皮细胞-半机细胞过渡 (EMT) 的过程.
研究的目的:
- 研究质细胞EMT在黑色素瘤进展中的作用.
- 探索Twist介导的EMT对黑色素瘤入侵和生存的影响.
- 为了确定潜在的分子机制改变了黑色素瘤细胞的行为.
主要方法:
- 采用了斑马鱼黑色素瘤模型.
- 进行了人类细胞共同培养试验.
- 单细胞RNA测序 (scRNA-seq) 用于分析黑色素瘤细胞群.
主要成果:
- 状细胞中Twist的过度表达导致斑马鱼黑色素瘤模型的生存率提高.
- 在Twist过度表达质细胞的存在下,黑色素瘤的入侵减少了.
- 在Twist过度表达条件下,scRNA-seq发现了一个独特的,不那么侵入性的黑色素瘤细胞群.
- 特定的细胞-细胞相互作用 (jam3b-jam3b和pgrn-sort1a) 被提名为减少入侵的媒介.
结论:
- 瘤微环境 (TME) 内的角质细胞中的EMT可以抑制黑色素瘤的入侵.
- 由质细胞EMT驱动的细胞间相互作用的改变可能会限制黑色素瘤的传播.
- 准角质细胞EMT可能是黑色素瘤的新疗法策略.
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