细菌交叉养可以通过降低基因表达成本来促进基因保留
Ying-Chih Chuang1,2, Megan G Behringer3, Gillian Patton3
1Department of Biology, Indiana University, Bloomington, IN, USA.
bioRxiv : the preprint server for biology
|September 4, 2024
概括
微生物群落中的交叉养可以通过抑制基因表达来防止基因丢失,这与预期相反. 这项研究表明,当微生物共享像腺素这样的资源时,基因保留而不是损失.
科学领域:
- 微生物生态学 微生物生态学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 当微生物群落通过交叉养共享资源时,通常会有基因损失,因为这会降低基因保留的成本.
- 然而,如果基因只在需要时被表达,可以避免基因表达成本,这可能导致基因保留而不是损失.
研究的目的:
- 调查交叉养是否导致长期细菌共同培养中的基因丢失或保留.
- 检查腺素交叉养对大肠杆菌基因丧失和Rhodopseudomonas palustris*铁获取基因表达的影响.
主要方法:
- 已经建立了长期的 *Escherichia coli* 和 *Rhodopseudomonas palustris* 的共同培养.
- 监测E. coli中的氨酸合成基因和R. palustris中的铁消耗性 siderophore 基因,以检测突变和表达水平.
- 分析了700代的基因表达和突变积累.
主要成果:
- 大肠杆菌没有积累氨酸合成基因的突变,尽管氨酸与R. palustris交叉养.
- 在大肠杆菌中,纯氨酸合成基因表达在培养中较低,表明抑制和减少对基因丢失的选择性压力.
- *R. palustris*在共同培养中显示出减少的 siderophore 基因表达,具有罕见的突变,与突变普遍存在的单一培养不同.
结论:
- 交叉养并不普遍导致基因丢失;它可以通过抑制昂贵的基因表达来促进基因保留.
- 资源共享可以降低基因表达的成本,从而有利于微生物群落中的基因保留.
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