相关实验视频
Updated: Jun 14, 2025

05:46
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
Published on: April 9, 2014
17.8K
在长效卡博特格拉维尔的HIV血清转化与整合酶抵抗突变的第一例病例,用于例行护理中的预防
Catherine A Koss1, Monica Gandhi1, Elias K Halvas2
1University of California, San Francisco, San Francisco, California, USA.
Open forum infectious diseases
|September 4, 2024
概括
长效卡博特格拉维尔 (CAB-LA) 是有效的预防艾滋病毒. 这一案例显示了INSTI耐药性在CAB-LA上发展,强调了更快的HIV检测和持续获得预防方法的需要.
科学领域:
- 传染性疾病 传染性疾病
- 病毒学 病毒学
- 药理学 药理学是指药理学的学科.
背景情况:
- 长效卡博特格拉维尔 (CAB-LA) 是预防艾滋病毒的高效暴露前预防 (PrEP).
- 临床试验已经注意到使用CAB-LA.的人的HIV诊断延迟和整合酶链转移抑制剂 (INSTI) 耐药性.
- 本报告详细介绍了在常规临床使用CAB-LA时出现的第一个具有INSTI耐药性的HIV感染病例.
研究的目的:
- 在接受长效卡博特格拉维尔 (CAB-LA) 常规临床护理的患者中报告第一个具有整合酶链转移抑制剂 (INSTI) 耐药性的HIV感染病例.
- 为了调查诊断挑战,抗逆转录病毒药物水平,以及在CAB-LA使用期间与艾滋病毒感染相关的耐药性模式.
主要方法:
- 该SeroPrEP研究招募了在美国接受PrEP期间感染艾滋病毒的个人.
- 使用单基因组测序 (SGS) 识别了完整长度HIV-1整合酶的耐药性突变.
- 血和头发中的卡博特格拉维尔度用液体染色学-并联质谱学量化.
主要成果:
- 一名23岁的个人在重新启动CAB-LA后发展出具有INSTI耐药性的HIV感染 (Q148R突变),尽管HIV检测和注射之间有20天的间隔.
- 敏感的研究分析检测到卡博特格拉维尔耐药性,而商业基因型定型失败了.
- 在诊断后观察到高血卡博特格拉维尔水平,表明坚持,但出现了耐药性.
结论:
- 这一案例强调了敏感测试对于检测CAB-LA患者INSTI耐药性的重要性.
- 艾滋病毒检测和CAB-LA启动的加速途径对于优化急性艾滋病毒检测和最大限度地降低耐药性风险至关重要.
- 无论保险状况如何,确保对CAB-LA的持续接入对于减少该人口中艾滋病毒感染至关重要.
更多相关视频
相关概念视频
Retrovirus Life Cycles
45.8K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.8K
Viral Mutations
32.2K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.2K
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K

