库尔库明基托桑微球通过IGF2BP1-介导的m6A修饰LRP5在性结肠炎中调节Th17/Treg平衡
Ling Chen1,2, Yanru Xiang3,2, Shirong Zhong4
1Traditional Chinese Medicine Department, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, China.
Iranian journal of basic medical sciences
|September 4, 2024
概括
黄微球 (CCM) 通过重新平衡T辅助细胞17 (Th17) 和调节性T (Treg) 细胞,有效治疗性结肠炎 (UC). 这通过IGF2BP1介导的m6A修饰发生,为UC提供了新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,其特征是T辅助细胞17 (Th17) 和调节性T (Treg) 细胞的不平衡.
- 黄素基托桑微球 (CCM) 在调节UC中关键的Th17/Treg平衡中的作用仍然在很大程度上未被探索.
研究的目的:
- 在小鼠模型中研究CCM对UC的治疗效果.
- 阐明CCM影响Th17/Treg细胞平衡的潜在机制.
- 确定参与CCM免疫调节作用的关键分子参与者.
主要方法:
- 使用德克斯硫酸盐 (DSS) 建立UC小鼠模型.
- 用CCM治疗UC小鼠,并通过流细胞计量评估Th17/Treg细胞种群.
- 研究基因表达变化 (Foxp3,RORγt,IL-10) 和与RNA修饰相关的基因,重点研究IGF2BP1和LRP5.5.
主要成果:
- CCM显著改善了UC症状,减少了Th17细胞,增加了Treg细胞.
- CCM调节了关键基因表达,包括增加了Foxp3和降低了RORγt和IL-10.
- IGF2BP1被确定为一个关键的调解者,其过度表达抑制了CCM的治疗效果;IGF2BP1通过m6A修饰准LRP5.
结论:
- 通过IGF2BP1介导的m6A修饰,CCM通过重新平衡Th17/Treg细胞来有效地治疗UC.
- IGF2BP1-LRP5通路是CCM在UC中的治疗作用的新目标.
- 这些发现为UC治疗开发提供了新的途径.
更多相关视频
相关概念视频
Drugs for Treatment of Ulcerative Colitis in IBD
130
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
130
T Cell Types and Functions
963
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
963
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
145
Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
145
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
328
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
328
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
98
Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...
98
Chronic Bowel Disorders: Introduction
414
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
414


