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Olfr2 阳性巨细胞源于单细胞,在位增殖,并呈现出一种亲炎性泡状的表型

Sujit Silas Armstrong Suthahar¹, Felix Sebastian Nettersheim¹, Ahmad Alimadadi^1,2

¹Division of Inflammation Biology, La Jolla Institute for Immunology, 9420 Athena Cir, La Jolla, CA 92037, USA.

Cardiovascular research

|September 4, 2024

概括

大动脉中的嗅觉受体2 (Olfr2) +巨细胞起源于单细胞,并在动脉样硬化早期积累. 这些细胞增殖并促进炎症,影响疾病的进展.

科学领域:

心血管生物学心血管生物学
免疫学免疫学免疫学
动脉样硬化研究动脉样硬化研究

背景情况:

单核细胞,包括单核细胞和巨细胞,是动脉样硬化的关键参与者.
动脉样硬化动脉壁含有不同的单细胞和巨细胞子集,具有不同的功能.
嗅觉受体2 (Olfr2) 存在于血管巨细胞中,其耗尽会影响动脉样硬化.

研究的目的:

在动脉样硬化中研究Olfr2-表达血管巨的功能表型和起源.
了解Olfr2+巨细胞在动脉样硬化的发展和进展中的作用.
定义Olfr2+巨细胞的分子特征和细胞行为.

主要方法:

用Apoe-/-小鼠食西方饮食来研究动脉样硬化.
采用质量细胞计和流细胞计用于细胞表征.
进行了采用转移实验和RNA测序 (RNA-seq) 以追踪细胞起源和识别分子签名.
分析了人类动脉样硬化斑块中的基因组丰富.

主要成果:

在动脉样硬化小鼠的大动脉中,Olfr2+巨细胞迅速增加.
这些巨细胞表达出促炎性细胞因子和化学因子.
收养转移证实Olfr2+单细胞在主动脉中分化为巨细胞.
Olfr2+巨体呈现出增加的增殖和促炎性标志物表达.
在人类动脉样硬化斑块中观察到类似的分子特征.

结论:

Olfr2+巨细胞起源于单细胞,并在动脉样硬化早期积累.
这些细胞分化为特定的巨子集 (MacAir,Trem2Gpnmb泡巨).
Olfr2+巨细胞具有增殖和促炎能力,显著影响动脉样硬化斑块的发展.

关键词:

动脉样硬化是一种动脉样硬化.炎症炎症是一种炎症.巨细胞是一个巨细胞.嗅觉受体是一种嗅觉受体.在奥尔弗雷德2 一个单细胞RNA-seqq.

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