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克里斯普尔/dCas13 (Rx) 衍生RNAN6-甲基氨酸 (m6A) 在植物中的动态修饰

Lu Yu¹, Muna Alariqi¹, Baoqi Li¹

¹National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|September 4, 2024

概括

新的工具精确地编辑植物RNA中的N6-甲基氨酸 (m6A). 这些m⁶A编辑器增强了根生长和抗干旱能力,显示了作物改善的潜力.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生物技术是生物技术.

背景情况:

N6 - - 甲基氨酸 (m6A) 是一种关键的mRNA修饰,调节植物生长.
缺乏有效的工具来精确地测量单个植物转录的修改.

研究的目的:

开发可编程工具,用于在工厂中进行有针对性的m⁶A沉积和去除.
调查植物基因调节和特征中的m⁶A修饰的功能作用.

主要方法:

结合的CRISPR/dCas13(Rx) 与甲基转移酶GhMTA (TME) 或甲基转移酶GhALKBH10 (TDE) 的组合.
应用TME和TDE编辑器来定位内转录GhECA1和GhDi19.
量化m⁶A水平的变化和编辑植物中评估的表型影响.

主要成果:

开发了TME和TDE编辑器,以便在0-46 nt窗口内高效地进行修改.
TDE降低了m⁶A水平的24%-76%;TME增加了m⁶A丰富的1.37到2.51倍.
用TME准GhDi19显著增加了根的长度和抗旱能力.

结论:

可编程的m⁶A编辑器 (TME和TDE) 对于植物的向RNA修改是有效的.
一个修改在调节 GhECA1 和 GhDi19 mRNA 中发挥了相反的作用.
这些m⁶A编辑器为功能研究和作物改进提供了一个强大的平台.

关键词:

克里斯普尔/dCas13 (Rx) 的使用 RNA m6A 修饰的改变棉花植物棉花植物耐旱耐受性耐旱耐受性

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