创新的可持续生物反应器在一个颗粒配方的Trichoderma asperelloides
Lucas Guedes Silva1, Renato Cintra Camargo2, Gabriel Moura Mascarin3
1Faculdade de Ciências Agronômicas, Departamento de Proteção Vegetal, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, SP, 18610-307, Brazil.
Applied microbiology and biotechnology
|September 4, 2024
概括
这项研究开发了一种基于大米面的新型配方,用于Trichoderma asperelloides (T. asperelloides) 的生物控制剂,创建了一个"生物反应器中的颗粒"系统. 颗粒配方提供了延长的保质期和有效控制对Sclerotinia sclerotiorum.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
背景情况:
- 菌类生物控制剂的进步需要使用农业工业副产品进行具有成本效益的大量生产.
- 目前的方法通常依赖于谷物谷物,需要经济发展的替代方案和稳定的配方.
- 斯克莱罗提尼亚 (Sclerotinia sclerotiorum) 是一种破坏性病原体,在全球范围内影响着众多的二角作物.
研究的目的:
- 开发一种基于大米面的创新配方,用于Trichoderma asperelloides CMAA1584.4.
- 在颗粒中创建一个微生物反应器系统,用于现场冷却和延长保质期.
- 为了评估该配方对Sclerotinia sclerotiorum的疗效.
主要方法:
- 用米粉,农业工业副产品,作为大规模生产的主要原料.
- 优化发酵条件,重点关注含量 (0.1% w/w) 和源 (水解酵母).
- 通过挤出和空气干燥开发颗粒式配方 (GControl,GLecithin,GBreak-Thru,GBentonite,GOrganic compost+Break-Thru),评估分泌物,稳定性和有效性. 通过挤出和空气干燥开发颗粒式配方 (GControl,GLecithin,GBreak-Thru,GBentonite,GOrganic compost+Break-Thru),评估分泌物,稳定性和有效性.
主要成果:
- 通过用水解酵母进行优化粉发酵,实现了高性产量 (2.6 × 10^9 CFU/g).
- 颗粒配方GBentonite和GOrganic堆肥+Break-Thru在冷藏储存下显示可持续24个月.
- 配方有效地抑制了Sclerotinia sclerotiorum发芽 (79.293.7%的相对抑制) 和硬化菌,即使在冷藏24个月后也是如此.
结论:
- 使用大米粉开发了一种环保,没有废物的Trichoderma conidia颗粒配方.
- "生物反应器中的颗粒"系统增长了生物杀虫剂的保质期,并保持了对Sclerotinia的有效性.
- 这种方法提供了一种可持续且具有成本效益的方法来生产和应用真菌生物控制剂.
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