相关实验视频
Updated: Jun 14, 2025

08:18
Induction and Validation of Cellular Senescence in Primary Human Cells
Published on: June 20, 2018
16.8K
通过管理细胞衰老来强化皮肤病皮肤的新策略
Journal of drugs in dermatology : JDD
|September 4, 2024
概括
微量服用亚洲和曼德利酸有效地减少了老化细胞和皮肤炎症,通过逆转皮肤衰老来提供一种新的皮肤质疏松症 (DP) 管理方法.
科学领域:
- 皮肤病学 皮肤病学
- 细胞生物学 细胞生物学
- 衰老研究研究 衰老研究
背景情况:
- 皮质疏松症 (DP) 的特征是由于纤维细胞功能障碍,细胞外基质 (ECM) 退化和皮肤-表皮结节的削弱而导致皮肤变薄和脆弱.
- 细胞衰老,特别是在乳头皮肤纤维细胞中,是对DP的ECM变化的重要但未被认可的贡献者.
- 衰老细胞释放炎症性细胞因子,加剧皮肤衰老和脆弱性.
研究的目的:
- 为了研究微剂量的亚洲菌和曼德利酸对皮肤老化的细胞的影响.
- 评估这些活性剂在减少炎症和改善皮肤衰老标志物的有效性.
- 探索针对细胞衰老的潜力,以管理皮肤病.
主要方法:
- 人类角质细胞和纤维细胞被培养,以确定非细胞毒性亚洲菌 (0.005%) 和曼底酸 (0.05%) 的微剂量.
- 进行RNA测序以分析治疗后的基因表达变化.
- 用光损伤皮肤的ex vivo模型来评估衰老细胞的减少 (p16INK4a) 和评估基底表皮细胞中的JAG/NOTCH信号.
主要成果:
- 试验室研究显示了协同效应,降低了老化相关分泌表型 (SASP) 细胞因子和炎症.
- 活体分析显示,乳头皮肤中的衰老细胞显著减少 (P<0.05).
- 在基底表皮细胞中观察到JAG/NOTCH表达的显著增加 (P<0.001),表明信号通路的调制.
结论:
- 微剂量中亚洲菌和曼德利酸提供了一种新的方法来逆转皮肤细胞衰老,并促进抗炎环境.
- 基因表达分析和体外模型验证了这种微剂量策略在治疗皮肤质疏松症方面的有效性.
- 这种方法为治疗皮质疏松症提供了一个有前途的新方向,通过准皮肤纤维细胞中的细胞衰老.
相关概念视频
Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
The Effect of Aging on Tissues
2.1K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.1K
Aging
40
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
40

