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强大的双乳液用于多色光激活细胞分类.

Yun Ding1, Giada Zoppi2, Gaia Antonini2

  • 1Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093 Zürich, Switzerland.

Analytical chemistry
|September 4, 2024
PubMed
概括
此摘要是机器生成的。

本研究介绍了使用商业光激活细胞分类器 (FACS) 和双重乳液进行高通量细胞分类的简单工作流. 这种方法可以有效地选免疫细胞激活的多细胞相互作用.

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科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 多细胞生物依赖于细胞-细胞相互作用,例如T细胞受体 (TCR) 与T细胞和抗原呈现细胞 (APC) 之间的抗原相互作用.
  • 光激活滴滴分类 (FADS) 是一种高通量选细胞相互作用的方法,但当前的仪器在通量和操作复杂性方面存在局限性.
  • 商业光激活细胞分类器 (FACS) 提供高速度,灵敏度和多重化,但在滴滴分类应用中未得到充分利用.

研究的目的:

  • 开发一种普遍适用的,易于实施的工作流程,用于生成双重乳液,并使用商用FACS仪器进行多色细胞分类.
  • 通过利用商业FACS机器的能力,克服现有的FADS技术的局限性.

主要方法:

  • 开发一种新的工作流程,用于生成与商业FACS仪器兼容的双重乳液.
  • 在产生的双重乳液中实施多色细胞分类.
  • 验证工作流程的效率和适用于选细胞相互作用的适用性.

主要成果:

  • 实现了超过90%的双乳液检测率.
  • 启用了多细胞封装和高通量免疫细胞激活分类.
  • 为先进的微流体应用展示了一种简单且具有成本效益的方法.

结论:

  • 本文所介绍的滴滴分类策略为细胞生物学实验室提供了一个普遍适用的,易于实施的工作流.
  • 与现有的FADS技术相比,这种方法提高了分析吞吐量,并简化了操作.
  • 该方法提供了以最小的努力和成本访问先进的微流体工具箱,促进了细胞与细胞相互作用和免疫反应的研究.