鱼提取物通过降低SGLT1和GLUT2在Caco-2细胞中的表达来抑制葡萄糖的摄取
Taichi Sato1, Katsuya Narumi1,2, Rin Taguchi1
1Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University.
Biological & pharmaceutical bulletin
|September 4, 2024
概括
富含脱氧核酸单酸盐 (dNMP) 的鱼粉提取物 (SME) 降低了肠道葡萄糖的吸收. SME和dNMPs降低葡萄糖载体SGLT1和GLUT2的表达,其中dTMP是关键.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 鱼取物 (SME) 含有脱氧核酸单酸盐 (dNMPs),可能提供抗肥胖益处.
- 肠道葡萄糖吸收主要由依赖的葡萄糖载体1 (SGLT1) 和葡萄糖载体2 (GLUT2) 介导.
研究的目的:
- 研究SME对肠道葡萄糖载体 (SGLT1和GLUT2) 功能和表达的影响.
- 阐明SME抑制葡萄糖吸收的机制.
主要方法:
- 差异化Caco-2细胞被用于研究SME对葡萄糖载体表达和功能的影响.
- 进行了放射性标记的葡萄糖吸收试验 ([14C]-甲基-α-D-葡萄皮化物和[3H]-2-脱氧-D-葡萄糖).
- 通过mRNA和蛋白质试验分析了SGLT1,GLUT2,HNF-1α和HNF-1β的表达水平.
主要成果:
- 治疗SME显著降低了SGLT1和GLUT2mRNA和Caco-2细胞中的蛋白质表达.
- SME和dNMP混合物降低了葡萄糖吸收,其中2'-脱氧胺5'-单酸盐 (dTMP) 显示起了显著的作用.
- SME和dNMPs调节了转录因子HNF-1α和HNF-1β的表达,这些转录因子调节SGLT1和GLUT2.
结论:
- 鱼粉提取物通过降低SGLT1和GLUT2表达的调节,有效地降低了肠道葡萄糖的吸收.
- 脱氧核酸单酸盐,特别是dTMP,是SME抑制葡萄糖运输机制的关键组成部分.
- SME的作用涉及HNF-1α和HNF-1β的调节,影响葡萄糖载体表达和随后的葡萄糖吸收.
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