基于单分子光和Hi-C方法的全球分布的宏观虫的两个近染色体级基因组
Wei Liu1, Xiaofei Shi1, Yingli Cai2
1The Germplasm Bank of Wild Species & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Scientific data
|September 4, 2024
概括
用先进的测序揭示了对Discinaceae家族 (Pezizales) 的基因组洞察力. 这项研究为了解这些重要的宏观真菌的进化和生态学提供了基础.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 迪斯奇纳科家族 (Discinaceae) 是一个分布在全球的宏观菌群,属于Pezizales系.
- 对Discinaceae的基因组数据有限,这阻碍了对其进化,发展和生态作用的理解.
研究的目的:
- 为了对Gyromitra esculenta和Paragyromitra xinjiangensis进行全面的基因组分析.
- 阐明这些物种的基因组景观,进化模式和生态特征.
主要方法:
- 利用第三代单分子光和Hi-C辅助的基因组组装.
- 进行了比较基因组分析.
- 通过使用BUSCO (基准测量通用单拷贝正义学) 评估基因组完整性.
主要成果:
- 组装了G. esculenta (47.10 Mb,23个支架) 和P. xinjiangensis (48.20 Mb,22个支架) 的基因组.
- 确定了10,438和11,469个编码蛋白,具有高的功能注释率 (>94%).
- 揭示了保存的蛋白质完整性 (98.71%和98.89%),确定了异质的生命周期特征,独特的染色体进化模式和潜在的食营养.
结论:
- 这项研究为Discinaceae家族提供了关键的基因组资源.
- 这些发现为Pezizales物种的进化轨迹,营养模式和生态功能提供了洞察力.
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