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东方栗黄黄蜂 (Dryocosmus kuriphilus) 的染色体层次的基因组组合

Bo Liu^1,2, Ye-Song Ren¹, Cheng-Yuan Su¹

¹Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Sciences, Central South University of Forestry and Technology, Changsha, China.

Scientific data

|September 4, 2024

概括

通过先进的测序,创建了入侵性栗子胆 (Dryocosmus kuriphilus) 的高质量基因组组. 此资源有助于研究其入侵性和栗子树的管理策略.

科学领域:

基因组学就是基因组学.
昆虫学昆虫学是一门学科.
植物病理学植物病理学

背景情况:

栗叶黄蜂 (Dryocosmus kuriphilus) 是一种来自中国的高度入侵性害虫.
它对全球的栗子树造成了重大损害,是欧洲检察官办公室的隔离对象.
了解它的遗传构成对于管理它的传播和影响至关重要.

研究的目的:

为了构建Dryocosmus kuriphilus的染色体级基因组组合.
为研究黄蜂的进化,遗传学和入侵性提供基础资源.
支持制定有效的管理策略来对抗这种害虫.

主要方法:

使用了PacBio长读数,Illumina短读数和Hi-C测序数据.
构建了一个染色体级别的组件,将结合物在10个伪染色体上.
使用BUSCO分析进行基因组完整性评估.

主要成果:

一个2.28 Gb的基因组组合,有14729个contigs和0.8 Mb的contigN50.
2.17Gb的结合体固定在10个伪染色体上,其N50支架为198.8Mb.
预测了24,086个编码蛋白质的基因,并确定了1.82 Gb的重复 (79.7%的基因组).

结论:

高质量的基因组组合为Dryocosmus kuriphilus研究提供了宝贵的参考资料.
促进了关于进化史,遗传结构,宿主-害虫相互作用和入侵性的研究.
促进针对这种入侵性害虫的有针对性的管理策略的制定.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.