酵母VAPs Scs2和Scs22是NVJ完整性和微核菌所需要的
Md Imran Nur Manik1, Most Naoshia Tasnin1, Tsuneyuki Takuma1
1Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
Biochemical and biophysical research communications
|September 4, 2024
概括
与VAMP相关的蛋白质 (VAPs) 对于核-真空结和微核来说至关重要,微核是一种在营养缺乏期间降解核部分的过程. 在长时间的营养缺乏下,酵母VAP对生存至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 微自和微核是细胞降解途径,发生在真空中.
- 微核选择性地降解核部分,涉及核-真空结 (NVJ).
- 这些过程是由营养饥饿诱导的,特别是通过拉帕素复合物1 (TORC1) 的目标失活.
研究的目的:
- 研究VAMP相关蛋白 (VAPs),Scs2和Scs22在微核的作用.
- 确定VAP对于核-真空结 (NVJ) 完整性和核蛋白质降解的必要性.
- 评估VAP突变在营养饥饿期间对酵母活力的影响.
主要方法:
- 利用芽生长酵母 (Saccharomyces cerevisiae) 作为一个模型生物体.
- 生成并分析VAP突变细胞 (scs2,scs22突变细胞).
- 评估了NVJ完整性,通过微核细胞的核蛋白降解,以及在营养饥饿条件下的细胞活力.
主要成果:
- 与VAMP相关的蛋白质 (VAPs) Scs2和Scs22对于保持核-真空结 (NVJ) 完整性至关重要.
- VAPs是核蛋白的微核菌降解所需的.
- 在长时间的营养饥饿期间,酵母VAP对细胞活力至关重要.
结论:
- 与VAMP相关的蛋白质在NVJ的结构完整性中起着至关重要的作用.
- VAP对于微核菌途径,特别是核子组件的降解是不可或缺的.
- 这项研究强调了VAP在酵母适应和在营养压力期间生存中的重要作用.
相关概念视频
01:28Yeast Signaling
14.5K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.5K
01:33Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
01:16In-vitro Mutagenesis
13.9K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.9K
01:18Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
01:27Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K