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转录组分析和全基因组识别脱水反应元素结合基因家族在寒冷应激下在中

Xiangwei Ma^1,2, Pengjin Zhu^3,4, Yingjun Du^1,2

¹Guangxi Subtropical Crops Research Institute , Nanning, 530000, China.

|September 4, 2024

概括

通过分析脱水反应元素结合基因 (DREB) 来研究果的抗寒性. 大多数AhDREB基因在抗寒性较低的基因中是下调的.

科学领域:

植物科学植物科学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

杰克水果 (Artocarpus heterophyllus Lam.) 是一种天然的水果. 易受低温损伤,影响生长.
识别抗寒基因对于培育改善的子品种至关重要.
脱水反应元素结合 (DREB) 基因家族在植物非生物应激反应中发挥着关键作用.

研究的目的:

为了研究DREB基因在果耐寒性中的作用.
为了比较"广西" (GX) 和"泰国" (THA) 麻品种的抗寒性.
为了确定关键的DREB基因,涉及到杰克果的寒冷应激耐受性.

主要方法:

使用叶绿素光学对抗寒冷的比较分析.
转录组测序用于分析基因表达特征.
对DREB家族的遗传学分析和基因结构分析.

主要成果:

在果基因组中发现了93个DREB基因,分为七组.
大多数已识别的AhDREB基因在抗寒性较低的"THA"菌株中表达下调.
定量实时PCR证实了"THA"中选择的AhDREB基因 (AhDREB12,AhDREB21,AhDREB29,AhDREB34) 的下调表达.

结论:

德雷布基因家族对于增强果的抗寒能力具有重要意义.
了解AhDREB基因调节,可以了解果对寒冷压力的反应.
这项研究奠定了杰克果对寒冷耐受性的遗传改进的基础.

关键词:

寒冷的压力是冷的压力.脱水反应元素结合 (DREB) 是一种表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式杰克果是一种果.

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Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...