相关实验视频
Updated: Jun 14, 2025

10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
24.3K
在棉花中识别和功能性表征双向基因对及其基因间区域
Jiangtao Yang1, Lihua Gao1, Xiaochun Zhang1
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory on Molecular Safety Assessment of Agri-GMO, MARA, Beijing, 100081, China.
BMC plant biology
|September 4, 2024
概括
研究人员在棉花中发现了1383个双向基因对,其中25个显示出促进体活性. 这一发现为改善转基因作物和理解基因进化提供了有价值的双向促进剂.
科学领域:
- 基因组学就是基因组学.
- 植物生物技术 植物生物技术
- 分子进化分子进化
背景情况:
- 改进转基因作物需要同时引入多个基因.
- 与单向促进剂相比,双向促进剂提供了高效的同时基因调节.
- 在Gossypium hirsutum TM-1中对双向基因对进行系统分析对于作物遗传改进至关重要.
研究的目的:
- 在Gossypium hirsutum TM-1中系统分析双向基因对.
- 探索这些基因的结构,功能和进化关系.
- 识别和验证用于作物育种的内源双向促进剂.
主要方法:
- 利用改进的搜索模型在棉花基因组中识别双向转录对.
- 进行基因结构和功能注释的系统分析.
- 进行过渡性表达测试以选基因间序列中的促进体活性.
主要成果:
- 在Gossypium hirsutum TM-1中识别了1383个双向转录对.
- 在30个随机选的跨基因序列中,25个中验证了促进子活性.
- 揭示了棉花亚种之间的双向基因的高同质性和保存的功能,以及双色球和单色球之间的功能.
结论:
- 确定了许多双向基因对,并验证了棉花中的促进子活性.
- 通过多基因共同转化为转基因育种提供了宝贵的双向促进者资源.
- 为基因工程和开发具有增强特征的新棉花品种提供了理论基础.
更多相关视频
相关概念视频
Cis-regulatory Sequences
9.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.8K
Epistasis Analysis
4.9K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.9K

