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通过使用16S rRNA基因测序来探索短期抗生素对儿童肠道微生物群的影响:一个病例控制研究

Yuhan Zhou¹, Xianglian Chen², Tongtong Wang²

¹Department of Pediatrics, The Fifth Affiliated (Zhuhai) Hospital of Zunyi Medical University, Zhuhai, 519100, China. Zhouyuhan4406@outlook.com.

|September 4, 2024

概括

抗生素的使用显著改变了儿童的肠道微生物群的多样性和功能,即使在短期治疗. 特定的抗生素类别会影响不同的微生物群体,为相关疾病提供潜在的生物标志物.

科学领域:

微生物学微生物学
儿科儿科儿科
药理学药理学是指药理学的学科.

背景情况:

抗生素的使用广泛,需要关注其副作用,特别是儿童.
了解抗生素引起的肠道微生物群变化对于解决相关疾病至关重要.

研究的目的:

分析抗生素治疗后儿童肠道微生物群的特征变化.
探索抗生素相关疾病的发病因子,并提供诊断/治疗见解.

主要方法:

招募了28名患有支气管肺炎的儿童,分为三个抗生素治疗组.
收集的便样本在抗生素治疗前和后 (3-5天).
利用16S rRNA基因测序和威尔科克森等级和和测试进行微生物群分析.

主要成果:

短期抗生素治疗显著改变了肠道微生物群的组成和多样性 (β多样性),尽管α多样性没有变化.
特定的抗生素差异抑制细菌类:梅洛西西林硫巴克坦 (Proteobacteria, Bacteroidetes, Verrucomicrobia), (Ceftriaxone) (Verrucomicrobia, Bacteroides) 和阿齐思罗米辛 (Fusobacteria, Actinobacteria, Proteobacteria, Verrucomicrobia). 这种细菌类的抗生素可以被分别抑制.
阿齐思罗米和氏显著影响了肠道微生物群的代谢功能,而梅洛西西林硫巴克坦没有显著影响.

结论:

抗生素治疗对儿童的肠道微生物群多样性和代谢功能产生深远的影响,即使在短时间内.
不同的抗生素类别对特定的微生物群落产生不同的影响.
确定了抗生素治疗后肠道微生物群的特定变化,这些变化可以作为诊断和管理抗生素相关疾病的生物标志物.

关键词:

16S rRNA 是一个16S rRNA.抗生素抗生素是一种抗生素.孩子孩子孩子孩子效应的效应影响的效应我们的肠道微生物群.

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