锡林NAC DOMAIN 1 (EjXND1) 通过负调节洛卡特水果中的EjHB1-EjPRX12模块来缓解冷诱导的化
Zihao Liang1, Yanna Shi2, Yiqing Huang1
1College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Journal of advanced research
|September 5, 2024
概括
洛卡特水果中冷诱导的化被EjXND1抑制,这是通过DNA甲基化调节的转录因子. 这一发现有助于开发修改低温调节 (mLTC) 等治疗方法,以提高水果质量.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 素的积累会对水果质量产生负面影响,特别是在对温度敏感的红肉中.
- 寒冷压力会触发木质化,这种过程会损害洛卡特水果的质量.
- 低温调节 (LTC) 是一种现有的方法来缓解寒冷伤害症状.
研究的目的:
- 为了研究控制洛卡特水果冷诱导化的分子机制.
- 为了确定瓜果的化途径中涉及的关键调节因素.
- 了解温度等环境因素如何影响红素生物合成.
主要方法:
- 转录组分析以确定候选基因.
- 在烟草和肝草中使用过渡性和稳定的转基因系统进行功能基因分析.
- 试验室测试包括蛋白质-蛋白质相互作用,双化酶和电泳运动转移测试 (EMSA) 以阐明调控机制.
主要成果:
- 鉴定出EjXND1,一个Xylem NAC域的转录因子,是洛卡特水果线性化抑制剂.
- EjXND1与素激活剂EjHB1相互作用,减少其激活EjPRX12促进体的活性.
- 在EjXND1的两个促进子区域中的DNA甲基化与其表达具有负相关性,影响激活剂结合.
结论:
- EjXND1是修改低温调节 (mLTC) 中的关键参与者,用于缓解冷诱导的化.
- EjXND1通过准EjHB1-EjPRX12模块来起作用,从而控制素生物合成.
- EjXND1的调节作用是由其促进区内DNA甲基化水平的动态变化所调节的.
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