下一代测序用于检测引起小儿社区肺炎的病原体 - - 一项系统性审查和元分析
Kiran Chawla1, Rosemary Shaji1, Nayana Siddalingaiah1
1Department of Microbiology, Kasturba Medical College Manipal, Manipal Academy of Higher Education Manipal. Karnataka, 576104, India.
Indian journal of medical microbiology
|September 5, 2024
概括
下一代测序 (NGS) 为诊断小儿社区获得性肺炎 (CAP) 提供了一种高度准确的方法. 这种先进的诊断方法改善了治疗结果,并优化了儿童的抗生素使用.
科学领域:
- 儿童传染病 儿童传染病
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 儿童的社区性肺炎 (CAP) 是一个重大的公共卫生挑战.
- 准确的病原体鉴定对于在儿科CAP中有效的抗生素治疗至关重要.
研究的目的:
- 评估下一代测序 (NGS) 的实用性,以准确诊断儿科CAP.
- 评估NGS指导治疗对CAP儿童治疗结果的影响.
主要方法:
- 一项系统性审查和元分析,将NGS与儿科CAP的传统诊断方法进行比较.
- 在2012-2023年期间搜索了主要的电子数据库 (PubMed/MEDLINE,Embase,Scopus,Web of Science)
- 包括专注于儿科CAP (<18岁) 的研究,使用NGS与传统诊断一起.
主要成果:
- 与传统方法 (26%-78.51%) 相比,NGS显示病原体检测率显著更高 (86%-100%).
- 在纳入研究中,以NGS发现为指导的治疗导致了83.33%的病例的临床改善.
- 分析显示,比率为2.39,有利于NGS获得更好的结果.
结论:
- 下一代测序,特别是元基因组测序,是儿科CAP的高度准确的诊断工具.
- NGS可以增强抗生素管理,改善患者的治疗结果,潜在地减轻抗生素耐药性.
- 建议在NGS方法和解释方面培训医疗保健专业人员,以便广泛采用.
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