一个新的合发酵系统,用于具有多样化的生物活动的低分子量香
Haoqing Sun1, Jialin Ni1, Guoshuai Yang1
1School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
International journal of biological macromolecules
|September 5, 2024
概括
这项研究开发了一种新型的合发酵系统,以有效地生产低分子重量的山口香糖 (LXG). 这种新方法提高了LXG的产量,并揭示了其抗氧化剂和益生菌的潜力,用于肠道健康应用.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 桑坦 (XG) 是一种具有工业应用的多功能外聚糖.
- 低分子量桑坦 (LXG) 提供了更好的性能,但面临着生产挑战.
- 现有的LXG降解方法是有限的.
研究的目的:
- 为生产LXG开发一个高效的合发酵系统.
- 为了表征用于LXG合成的内分-桑坦酶酶.
- 评估产生的LXG的生物活性和潜在应用.
主要方法:
- 来自Microbacterium sp.的内桑坦酶的表达 在Pichia pastoris的XT11.
- 使用豆芽汁对配发酵系统的优化.
- 生物反应器培养与受控参数 (pH 6.0, 1:3 接种比率).
- 描述LXG分子量和单糖化合物的组成.
- 对LXG抗氧化和益生菌性质的评估.
主要成果:
- 优化发酵实现了高的总糖 (12.12 g/L),降解糖 (5.32 g/L) 和内分植物酶活性 (1150.26 U/L).
- 产生的LXG分子量为1093Da,具有特定的单糖比.
- LXG表现出显著的抗氧化和益生菌作用,促进有益的肠道细菌生长.
结论:
- 结合发酵系统对LXG生产非常有效.
- LXG具有宝贵的生物活性特性,包括抗氧化剂和益生菌功能.
- 作为以肠道健康为重点的产品的功能成分,LXG显示出前景.
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