CRADD和cIAP1对抗性地调节远中caspase-9介导的亡
Meng Wu1, Yuan Chen1, Zihao Yuan1
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China; College of Marine Sciences, University of Chinese Academy of Sciences, Qingdao, China.
International journal of biological macromolecules
|September 5, 2024
概括
鱼类的caspase 9 (CASP9) 调节与人类不同. 鱼cIAP1降解了亲和活跃的CASP9,而CRADD增强了CASP9的活性并阻断了cIAP1的抑制,揭示了新的teleost亡控制.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 细胞亡研究研究
背景情况:
- 酶9 (CASP9) 是人类内在亡的一个关键启动剂.
- 人类的cIAP1会降解激活的CASP9,但不会降解它的前体.
- 远鱼的CASP9调节在很大程度上仍然没有被描述.
研究的目的:
- 用 (Paralichthys olivaceus) 作为模型,研究远鱼中CASP9的调节机制.
- 为了比较人体中观察到的CASP9调节与人体中观察到的调节.
主要方法:
- 鱼CASP9 (PoCASP9) 和cIAP1 (PocIAP1) 的表达和功能分析.
- 研究PoCASP9,PocIAP1和鱼CRADD (PoCRADD) 之间的相互作用.
- 评估参与PoCASP9调节的蛋白质体降解途径.
主要成果:
- PocIAP1通过蛋白酶体通路结合并降解亲和活性PoCASP9,与人类cIAP1.1不同.
- PoCRADD与亲和活性PoCASP9相互作用,增强细胞亡并增加细胞CASP9水平.
- PoCRADD通过破坏它们的相互作用来阻止PoCASP9的PocIAP1-介导抑制.
结论:
- 遥远的CASP9调节与人类机制有很大差异.
- 鱼CASP9受到直接的正 (PoCRADD) 和负 (PocIAP1) 调节,在激活前和后都起作用.
- 这些发现阐明了一种独特的脊椎动物亡控制系统,促进了对跨物种CASP9功能的理解.
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