相关实验视频

Updated: Jun 14, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

46.1K

快速的小规模纳米体辅助净化氨酸受体的冷-EM

Chenyao Li1, Katrien Willegems1, Tomasz Uchański1

  • 1Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels, Belgium; Structural Biology Brussels, Department of Bioengineering Sciences, VUB, Brussels, Belgium.

The Journal of biological chemistry
|September 5, 2024
PubMed
概括

这项研究引入了一种基于纳米体的新方法,用于从最小的蛋白质样本中快速净化氨酸受体 (RyRs). 这一突破使RyRs的高效结构研究成为可能,这对于理解和治疗相关疾病至关重要.

关键词:
运输的运输.冷电子显微镜的使用方法离子通道 离子通道 离子通道膜蛋白质是一种膜蛋白质.蛋白质净化 蛋白质净化氨酸受体的受体是什么?一个单域抗体.

更多相关视频

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella11:31

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Published on: November 30, 2018

7.4K
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.4K

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K