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两种用于传统实验室和RapidHITTM ID工作流程的简化牙样本准备方法

Morgan Eaton¹, M Shane Woolf¹, Samyuktha Pemmasani¹

¹Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA.

Journal of forensic sciences

|September 5, 2024

概括

简化牙科DNA提取方法提供了灾难受害者快速识别 (DVI). 低技术的牙准备与RapidHITTM ID分析相结合,可以产生完整的STR概况,帮助法医调查.

科学领域:

法医科学法医科学法医科学
遗传学是一个遗传学.
人类学是人类学.

背景情况:

灾难受害者识别 (DVI) 在发生大规模伤亡事件后至关重要.
人类牙残骸在软组织退化时提供了宝贵的核DNA来源.
传统的法医DNA工作流程需要经过训练的人员进行广泛的样本准备.

研究的目的:

评估牙DNA提取的简化样品制备协议.
为了比较传统的法医DNA工作流程与应用生物系统® RapidHITTM ID仪器对DVI的有效性.

主要方法:

经过两种简化协议,处理了10颗叶落牙.
样品被使用爪进行碎片化,用于RapidHITTM ID,或用咖啡机粉碎,用于传统的工作流程.
分析了短串联重复 (STR) 基因组检测和峰值高度比率 (PHRs).

主要成果:

在RapidHITTM ID (99.0%) 和GlobalFilerTM (99.8%) 之间观察到相似的STR等位基因检测率.
平均内异构细胞的PHRs是相似的:RapidHITTM ID (0.80) 和GlobalFilerTM (0.86).它们是相同的.
快速HITTM ID工作流标记了90%的样本进行审查,其中91%的等位基因通过了质量指标,而GlobalFilerTM则为100%.

结论:

通过简化,低技术的牙碎片,然后通过RapidHITTM ID分析,可以从老牙样本中生成完整的STR配置文件.
需要进一步的研究来完善挑战样本的协议,并使现场 DVI.
这些方法有望提高DVI流程的速度和可访问性.

关键词:

在DNA分析中,分析DNA.个人资料 DNA DNA 档案 RapidHIT® 识别系统的识别系统.灾难受害者身份识别 (DVI)快速的 DNA DNA 快速的 DNA 一个牙样本.

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