洞察GC-MS和UHPLC-Q-TOF/MS代谢学对来自不同加工技术的Radix Bupleuri挥发性和极性化合物的组成变异
Jiaxi Li1, Chengye Yan1, Jiahao Li1
1Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, China.
Phytochemical analysis : PCA
|September 5, 2024
概括
这项研究比较了使用不同方法加工的Radix Bupleuri (RB) 中的活性成分. 结果揭示了关键的化学标记物,支持RB及其加工形式的差异化临床使用.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分析化学 分析化学
- 传统中国医学 (TCM) 研究研究
背景情况:
- 了解加工对Radix Bupleuri (RB) 活性成分的影响对于TCM疗效至关重要.
- 开发用于TCM处理的强大的分析方法是一个重大挑战.
研究的目的:
- 建立一个全面的方法来分析在加工前和加工后RB的组成变化.
- 为了研究由于不同的加工技术而导致RB活性成分变化的趋势.
主要方法:
- 拉迪克斯·布普勒乌里 (Radix Bupleuri) (RB) 是用大米,大米葡萄酒和蜂蜜进行加工的.
- 使用超高性能液态染色学 (UHPLC) 和气态染色学 (GC) 与质谱学 (MS) 相结合.
- 为UHPLC-MS开发了一种全新的理论碎片离子光谱和信息依赖性获取相互认证 (SIMA) 方法,用于所有理论碎片离子光谱和信息依赖性获取.
主要成果:
- 30种极性和33种挥发性化合物被确定为化学标记物.
- 关键标记物包括II型赛可索,烯和脂肪酸.
- 这些已识别的化合物代表了RB药理学活动的潜在物质基础.
结论:
- 该研究为RB及其加工产品的临床差异化提供了基础.
- 开发的SIMA方法显示了对TCM加工成分的准确分析的希望.
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