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多G) 盒子:哺乳动物18S rRNA的功能元素,参与翻译

Dahao Wei¹, Zhangyu Mai², Xinan Li³

¹School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou, China.

|September 5, 2024

概括

在哺乳动物的18S rRNA中,一种新的Poly(G) 7box序列调节了翻译. 这种元素与延长因子eEF1D和蛋白质RPL32结合,抑制细胞翻译效率.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

细胞核核糖体小子单元 (40S) 包括18SrRNA和33种蛋白质,对于翻译至关重要.
18S rRNA具有独特的二次结构,对蛋白质合成至关重要.

研究的目的:

为了识别和描述哺乳动物18S rRNA中的新功能元素.
为了研究新发现的Poly(G) 7box序列在翻译调节中的作用.

主要方法:

生物信息分析以确定哺乳动物18SrRNA中的Poly(G) 7box序列.
在体外测试研究Poly(G) 7box与转化因子 (eEF1D) 和核糖体蛋白质 (RPL32) 的结合.
细胞转染实验,以评估Poly(G) 7box对细胞翻译效率的影响.

主要成果:

在哺乳动物的18S.rRNA中发现了一种新型的Poly(G) 7box序列,由七个关氨酸核酸组成.
多G) 7box形成了一个稳定的二次结构,能够与翻译延长因子eEF1D和核糖体蛋白RPL32.2结合.
转化Poly(G) 7box进入细胞导致了翻译效率的抑制.

结论:

在哺乳动物18S.rRNA中,Poly(G) 7box序列代表了一个重要的翻译调节功能元素.
与mRNA上的对应物不同,mRNA5'和3'未翻译区域上的Poly(G) 7不会影响mRNA翻译.

关键词:

在18S rRNA中.保存的序列是可以保存的.聚G) 7盒子核糖体核糖体核糖体核糖体翻译监管要素翻译监管要素

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