LIG1是一种新的膀癌预后标记:基于实验研究,机器学习和单细胞测序的证据
Ding-Ming Song1, Tong Shen1, Kun Feng1
1Department of Urology, Jinzhou Medical University, The First Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Frontiers in immunology
|September 5, 2024
概括
在膀癌 (BLCA) 中,DNA连接酶1 (LIG1) 的高调,与生存率差相关. 这项研究揭示了LIG1
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 膀癌 (BLCA) 是一种致命的疾病.
- 在瘤细胞中,DNA联酶1 (LIG1) 经常被删除.
- 在BLCA中LIG1的作用尚不清楚.
研究的目的:
- 研究LIG1在BLCA中的作用.
- 探索LIG1和临床病理因素之间的相关性.
主要方法:
- 分析了来自GEO存储库的基因表达数据.
- 使用Seurat,Lemma和WGCNA软件包进行差异表达和网络分析.
- 进行KEGG,GO和PPI丰富分析以确定枢纽基因和通路.
- 开发了一种机器学习的诊断模型,并分析了突变概况和生存数据.
- 进行功能实验以验证LIG1的作用.
主要成果:
- 在BLCA中,LIG1被上调,这与不良的生存率有关.
- LIG1参与DNA复制,细胞循环和p53信号通路.
- 在LIG1组之间观察到突变格局和免疫细胞透的显著差异.
- 功能性实验证实了LIG1在BLCA病变发生过程中的作用.
结论:
- 通过增强扩散,入侵和EMT,LIG1促进BLCA的进展.
- LIG1 作为膀癌的潜在预后生物标志物.
相关概念视频
03:18Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K