在中国,临床Candida auris的基因微演变,AMPHOTERICIN B敏感性降低
Sufei Tian1, Chen Rong1, Hailong Li1,2
1National Clinical Research Center for Laboratory Medicine, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Laboratory Medicine, the First Hospital of China Medical University, Shenyang, People's Republic of China.
Emerging microbes & infections
|September 5, 2024
概括
这项研究揭示了Candida auris在膀灌治疗期间如何演变降低安二B (AmB) 敏感性. 主要基因突变和脂质和DNA途径的表达变化驱动了这种抗真菌耐药性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在 *Candida auris* 中,全球安福特素B (AmB) 耐药性超过12%.
- 关于临床治疗和与AMB敏感性降低相关的微演变的数据有限.
- *C. auris* 是一个重要的新兴真菌病原体,具有很高的耐药性.
研究的目的:
- 在临床治疗期间,研究*C. auris*中AmB敏感性降低的微演变.
- 识别与AMB易感性降低相关的遗传和转录组特征.
- 阐明低度AmB膀灌与抗药性发展之间的关系.
主要方法:
- 从五名患者 (2019-2022) 收集了18个*C. auris*分离体.
- 进行临床数据挖掘,基因组和转录组分析.
- 确定了AmB.的最小抑制度 (MICs).
主要成果:
- 在 *ERG3* (AmB1) 和 *RAD2* (AmB2) 中发现了与 AmB 敏感性降低相关的突变.
- 在AmB1分离物中观察到与膜脂相关的基因和在AmB2分离物中观察到与DNA相关的基因的上调.
- 鉴定了五个关键基因 (*IFF9*, *PGA6*, *HGT7*, *HGT13*, *PRI32*) 在耐药菌株中表达变化.
结论:
- 在体内证明了*C. auris** AmB敏感性降低的微演变.
- 建立了低度AmB膀灌和耐药性的发展之间的联系.
- 提供了对潜在的抗真菌药物标和 *C. auris* 感染的临床标记的见解.
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