工程细胞延长促进了Shewanella Oneidensis的细胞外电子转移
Feng Li1, Huan Yu1, Baocai Zhang1
1Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|September 5, 2024
概括
工程电活性微生物 (EAM) 用于细胞延长显著提高了细胞外电子转移 (EET) 和功率输出. 这种方法提高了微生物燃料电池的性能和污染物降解能力.
科学领域:
- 微生物学 微生物学
- 生物电化学 生物电化学
- 合成生物学 合成生物学
背景情况:
- 电活性微生物 (EAM) 对生物电化学系统至关重要.
- 在EAM中,细胞外电子转移 (EET) 效率限制了它们的应用.
- 细胞形态,特别是长度,是影响EET的潜在因素.
研究的目的:
- 为了研究编程细胞延长对Shewanella oneidensis MR-1的EET的影响.
- 为提高功率密度和污染物处理能力设计EAM.
- 阐明细胞延长介导的ETE增强背后的分子机制.
主要方法:
- 用Shewanella oneidensis MR-1进行基因工程,以使用反感官RNA或分裂抑制剂来抑制细胞分裂.
- 电子生理学测量以量化输出电流和功率密度.
- 转录组分析以了解细胞变化.
- 基于定数感应的动态调节,以实现持续的生长和延长.
主要成果:
- 工程菌株表现出显著增加的细胞长度和输出功率密度.
- 细胞延长通过上调NADH氧化,内膜子池和c型细胞染色体来增强EET.
- 长长的细胞显示出增加的疏水性,促进生物膜的形成.
- 定数感应调节使得产生超延长细胞 (143.6μm) 成为可能,功率密度增加了3.41倍 (248.0mW m-2).
结论:
- 编程细胞延长是一种有效的策略,可以在EAM中增强EET.
- 这种方法为改善微生物燃料电池性能和生物修复提供了一个新的途径.
- 细胞延长和质量检测的组合提供了一个强大的方法来优化EAM功能.
相关概念视频
Electron Transport Chain Components
1.2K
The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
1.2K
Anoxygenic Photosynthesis
1.7K
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
1.7K
Stringent Response in E. coli
477
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
477
Deep Sea Microbial Ecology
42
The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches...
42
Bioreactor Controls-III
54
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
54


