在血中测定可纳的UPLC-MS/MS方法及其在墨西哥候选血病患者中的应用
Karla Paulina Valero Rivera1, Martin Magaña Aquino2, Julia Sagahón Azúa1
1Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, #6 Ave. Manuel Nava, San Luis Potosí, C.P.78210, México.
Bioanalysis
|September 5, 2024
概括
一种新的超高性能液态色谱法准确地量化了人体血中的可纳. 这种快速和选择性测定适用于监测候选血病患者,可能改善治疗结果.
科学领域:
- 分析化学 分析化学
- 药理动力学 药理动力学
背景情况:
- 精确量化抗真菌药物,如可纳对于治疗系统性真菌感染,如候选性血症至关重要.
- 现有的分析方法可能缺乏常规临床监测所需的速度或灵敏度.
研究的目的:
- 开发和验证一种快速而准确的分析方法,用于在人体血中量化可纳.
- 确保该方法符合美国食品和药物管理局 (FDA) 的指导方针.
主要方法:
- 超高性能液态染色学与双重质谱学 (UHPLC-MS/MS) 相结合.
- 简单的蛋白质沉,使用乙尼来制备样本.
- 在Acquity UHPLC HSS T3 列上进行异离子化.
主要成果:
- 该方法表现出极好的线性 (r2 > 0.999) 和在1-50μg/mL范围内稳定性.
- 日内和日间精度显示变化系数从1.4%到6.6%.
- 成功分析了来自候选血病患者的27个血样本.
结论:
- 开发的UHPLC-MS/MS方法是准确,精确和快速的,用于在人体血中量化可纳.
- 简单的样本准备和缩短的分析时间使其非常适合在成人候选血病患者的临床应用.
- 这种方法可以帮助优化可纳治疗,改善患者的治疗结果.
更多相关视频
06:14Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
Published on: October 15, 2017
8.3K
10:22Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
Published on: April 3, 2018
7.6K
相关概念视频
01:26Therapeutic Drug Monitoring: Drug Analysis Methods
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...