使用LC/ESI-HRMS在马尿中量化氧化的量化,以建立用于兴奋剂控制的消除概况
Hideaki Ishii1,2, Ryo Shigematsu1, Shunsuke Takemoto1
1Drug Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsuruta-machi, Utsunomiya, Tochigi, 320-0851, Japan.
Bioanalysis
|September 5, 2024
概括
奥西洛德罗斯塔特是一种禁止用于马的合成代谢剂,可以在尿液中检测到两周. 建议对其代谢物M1c和奥西洛德罗斯塔特进行监测,以有效控制马匹的兴奋剂.
科学领域:
- 马类药理学 马类药理学
- 兴奋剂控制科学科学 兴奋剂控制科学
- 分析化学是一种分析化学.
背景情况:
- 奥西洛德罗斯塔特是一种类合成药物,主要国际联合会禁止在赛马中使用.
- 准确的检测方法对于执行马术运动中的反兴奋剂法规至关重要.
研究的目的:
- 为了在马尿中建立osilodrostat的排泄概况.
- 调查用于兴奋剂控制的氧化及其代谢物M1c的可检测性.
主要方法:
- 使用经过验证的方法分析注射后的马尿样.
- 酶性水解,然后进行LC/ESI-HRMS分析.
- 用0.05 ng/ml的下限量化值 (LLOQ) 进行量化.
主要成果:
- 在服用后48至72小时内,水解后的奥西洛德洛斯塔特在尿液中可量化.
- 化酸及其代谢物M1c都被检测了长达两周的时间.
- 确认性分析检测到水解的奥西洛德罗斯塔特长达72小时.
结论:
- 建议在马类兴奋剂控制中监测化M1c和振.
- M1c提供了更高的检测能力,而振荡机参考材料对于确认至关重要.
关键词:
在 LC/ESI HRMSMS 中.合成代谢剂是一种合成代谢剂.兴奋剂控制是对抗兴奋剂的控制.消除特征 消除特征 消除特征马类的马类动物.旋转的状态 旋转的状态定量分析量化分析尿液 尿液 尿液更多相关视频
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