相关实验视频

Updated: May 6, 2026

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

人类角质细胞对4,4'-甲基二二酸盐-谷氨酸合物暴露的反应

Brandon F Law¹, Chen-Chung Lin¹, Justin M Hettick¹

¹Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA.

Xenobiotica; the fate of foreign compounds in biological systems

|September 5, 2024

概括

通过皮肤接触4,4'-甲基二二酸盐 (MDI) 可能导致职业喘. 皮肤细胞中的基因和蛋白质表达变化表明免疫和炎症途径参与了MDI-OA的发展.

科学领域:

职业健康职业健康职业健康
免疫学免疫学免疫学
毒理学毒理学毒理学

背景情况:

工作场所暴露于二酸盐,如4,4-甲二二酸盐 (MDI),是已知的职业喘 (MDI-OA) 的原因.
基于MDI-OA的精确生物机制仍在调查中,越来越关注皮肤暴露的作用.

研究的目的:

为了研究暴露于MDI-Glutathione结合物 (MDI-GSH) 后人类角质细胞的分子变化.
通过检查基因和蛋白质表达来评估皮肤暴露在MDI-OA发展中的潜在作用.

主要方法:

使用基因表达 (RT-qPCR) 和蛋白质组学 (HPLC-MS/MS) 分析培养的人类HEKa角质细胞,暴露于MDI-GSH.
分析了RNA和蛋白质表达特征的变化,以确定分子变化.

主要成果:

在39个测试的基因中,RT-qPCR揭示了9个升级调节的候选基因.
蛋白质组分析确定了18540种蛋白质,其中60种在MDI-GSH暴露细胞中显示出统计学上显著的差异性表达.
不同表达的蛋白质表明免疫和炎症通路的激活.

结论:

这些发现支持了皮肤MDI暴露是MDI-OA发展的重要因素的假设.
蛋白质组和基因表达数据表明,多种先天性和适应性免疫,以及炎症途径都与MDI-OA病原发生有关.

关键词:

4-甲二二酸 (MDI) 是一种人类角质细胞细胞.异酸盐是一种异酸盐.没有标签的定量蛋白质组学.职业性喘 (OA) 是一种

更多相关视频

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Published on: August 10, 2019

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Published on: September 26, 2022

相关概念视频

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...

Hypersensitivity Reactions: Delayed Hypersensitivity Reactions

Hypersensitivity Reactions: Delayed Hypersensitivity Reactions

Delayed-Type Hypersensitivity (DTH), or Type IV hypersensitivity, is a cell-mediated immune response. It occurs when T cells, rather than antibodies, mediate a reaction to specific antigens. It is characterized by a delayed onset (1-2 days) and involves the recruitment of macrophages to the inflammation site.The initiation of a DTH response begins with the sensitization of T cells. During this phase, which lasts at least 1-2 weeks, antigen-specific T cells are activated, clonally expanded, and...

Bioactivation and Tissue Toxicity

Bioactivation and Tissue Toxicity

Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...