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删除PAB1的类效应:在酵母蛋白质组,转录组和翻译组中发生了广泛的变化

Kotchaphorn Mangkalaphiban¹, Robin Ganesan¹, Allan Jacobson¹

¹Department of Microbiology, UMass Chan Medical School, Worcester, Massachusetts, United States of America.

|September 5, 2024

概括

细胞质多A结合蛋白 (PABPC) 对酵母基因表达至关重要. 它的缺失会破坏翻译,mRNA衰变,并导致广泛的细胞缺陷,突出其重要的调节作用.

科学领域:

分子生物学分子生物学
酵母遗传学酵母遗传学
转录后条例转录后条例转录后条例

背景情况:

细胞质多A结合蛋白 (PABPC) 在mRNA处理中发挥作用.
PABPC参与翻译启动,终止和mRNA衰变.

研究的目的:

调查PABPC在转录后控制中的直接和间接作用.
分析PAB1基因删除对酵母蛋白质和转录基因的影响.

主要方法:

质谱测量以评估蛋白质丰富度.
RNA-Seq和Ribo-Seq用于分析转录组变化和翻译.
对缺乏PAB1基因 (pab1Δ) 的酵母细胞进行分析.

主要成果:

删除Pab1导致酵母蛋白质和转录蛋白质的显著变化.
在 pab1Δ 细胞中观察到翻译启动和终止的缺陷.
mRNA稳定和无意义的编码子读透表明广泛的转录后失调.

结论:

帕布1的缺失对多个转录后过程具有类效应.
丧失PABPC功能严重破坏细胞调节.
间接影响,如减少发起因子,有助于pab1Δ表型.

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