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在手术后的认知功能障碍中,lncRNA MEG3通过与EZH2和YTHDC1的相互作用对烧伤的功能作用

Zijian Ma¹, Haifei Niu², Haiqi Qi¹

¹Department of Anesthesiology, South area of the Affiliated Hospital of Chengde Medical College, Chengde, Hebei 067055, China.

Brain research bulletin

|September 5, 2024

概括

长非编码RNA MEG3 抑制神经炎症,并通过抑制NLRP3炎症酶途径,防止手术后认知功能障碍 (POCD). 这一发现为POCD治疗提供了潜在的治疗点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

手术后认知功能障碍 (POCD) 的机制尚不清楚,限制了有效的治疗方法.
神经炎症诱导的NLRP3烧途径有助于POCD的发展.
长非编码RNAMEG3在神经损伤和烧死中的作用需要进一步调查.

研究的目的:

在sevoflurane诱导的POCD中研究lncRNA MEG3的分子机制.
阐明MEG3在调节海马体神经元中的NLRP3炎症和热的作用.
探索MEG3作为POCD的潜在治疗点.

主要方法:

在体外和体外的sevoflurane诱导的POCD模型.
定量实时PCR (qRT-PCR),西斑,ELISA和基因和蛋白质表达的组织学染色.
细胞活力 (CCK-8) 和损伤 (LDH) 测试,莫里斯水迷宫 (MWM) 测试认知功能.
RNA免疫沉 (RIP) 和染色体免疫沉 (ChIP) 用于验证分子相互作用.

主要成果:

塞沃兰在小鼠中降低了MEG3和热的标志物.
过度表达MEG3可以缓解sevoflurane引起的认知缺陷和热.
通过EZH2/YTHDC1相互作用减少mRNA稳定性,MEG3降低了NLRP3的表达.

结论:

通过招募EZH2/YTHDC1.1,MEG3可以抑制NLRP3炎症酶和海马神经元灭.
MEG3在调节POCD方面发挥着至关重要的作用.
对于治疗POCD而言,MEG3是一个有前途的治疗标.

关键词:

在 EZH2 中使用.在MEG3中,MEG3是MEG3,MEG3是MEG3.手术后的认知功能障碍热灭菌的发生.在 YTHDC1 中.

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