第三类原蛋白调节在伤口愈合期间的矩阵架构和机械感知
Daniel C Stewart1, Becky K Brisson1, William K Yen1
1Department of Clinical Sciences & Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
The Journal of investigative dermatology
|September 5, 2024
概括
第三类原蛋白 (COL3) 是皮肤愈合和痕形成的关键. 较低的COL3水平通过改变细胞行为和原结构来促进痕,提供新的伤口管理策略.
科学领域:
- 生物医学科学 生物医学科学
- 皮肤病学 皮肤病学
- 再生医学是一种再生医学.
背景情况:
- 产后伤口愈合通常会导致痕形成,损害组织结构和功能.
- 尽量减少痕的形成,同时促进有效的愈合是伤口管理的关键目标.
研究的目的:
- 研究III型原蛋白 (COL3) 在调节皮肤伤口愈合,再上皮化和痕形成中的作用.
- 定义与痕宽容愈合相关的原结构特征,以及 COL3 如何影响它.
- 探索COL3缺乏如何影响细胞机制感知和亲纤维细胞信号传递.
主要方法:
- 使用多种小鼠皮肤伤口模型,包括COL3丰富,痕宽容和COL3缺陷 (有条件淘汰) 系统.
- 在愈合的伤口中分析了原纤维结构,特别是对齐和延长.
- 评估细胞机制感知和整合素参与 (整合素α11) 作为对COL3水平的反应.
主要成果:
- 确定了一种痕宽容的纤维状原蛋白结构,其特征是延长的异型纤维,这种纤维以剂量依赖的方式被COL3抑制.
- 证明COL3缺乏会改变细胞机制感知,导致机械活跃的表型,即使没有增加矩阵刚度.
- 显示了 COL3 缺乏细胞中益菌整蛋白 α11 的调节和参与.
结论:
- 第三种类型的原蛋白 (COL3) 是皮肤伤口愈合的关键调节者,影响着矩阵结构和细胞反应.
- 通过改变原蛋白对齐和通过整合素α11.11增强细胞机械传导,COL3缺乏会促进痕形成.
- 了解COL3的作用可能会导致新的临床策略,用于无痕伤口愈合.
相关概念视频
Phases of Wound Repair
5.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.9K
Role of Matrix Metalloproteases in Degradation of ECM
2.3K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.3K
Cell-matrix's Response to Mechanical Forces
2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.6K
Extracellular Matrix
2.8K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
2.8K
The Extracellular Matrix
8.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
8.9K
Structural Protein Function
27.5K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.5K


