胆基离子液体的细胞毒性和细胞膜相互作用:比较氨基酸,酸和酸离子
Mohamad El Mohamad1, Qi Han1, Brendan Dyett1
1School of Science, STEM College, RMIT University, 124 La Trobe Street, Melbourne, VIC, 3000, Australia.
Chemosphere
|September 5, 2024
概括
这项研究表明,生物相容离子液体 (IL) 的毒性和溶血活性取决于离子性和溶液pH值. 基于胆的IL与细胞膜的相互作用不同,影响流动性和结构.
科学领域:
- 生物相容的离子液体
- 细胞毒理学 细胞毒理学
- 膜生物物理学 膜生物物理学
背景情况:
- 离子液体 (IL) 越来越多地用于生物医学领域,但它们的毒性概况需要进一步研究.
- 了解影响生物相容IL细胞毒性的因素对于安全应用至关重要.
- 基于胆的IL是一种有前途的生物相容IL类,具有潜在的生物医学应用.
研究的目的:
- 为了研究13种基于胆的ILs的细胞毒性和溶血活性.
- 探索IL特性 (离子疏水性,pH) 和它们的生物效应之间的关系.
- 使用生物物理技术阐明IL-膜相互作用的机制.
主要方法:
- 使用四个人类细胞系 (HDF,A431,HeLa,AGS) 的细胞毒性测定.
- 血液溶解活性评估.
- 石英晶微平衡与消散 (QCM-D) 监测IL-膜相互作用.
- 总内部反射光 (TIRF) 显微镜用于膜结构变化.
主要成果:
- 细胞毒性和溶血活性与阴离子疏水性和IL溶液pH相关.
- 更有毒的ILs通过QCM-D与模型细胞膜 (DOPC) 产生更大的相互作用.
- TIRF显微镜显示了膜脂质的重新排列,孔隙形成 ([Cho][Ac]) 和双层破坏 ([Cho][Ge]).
结论:
- 阴离子水性和pH值是基于胆的IL毒性的关键决定因素.
- ILs与细胞膜相互作用,导致与其毒性相关的结构变化.
- 调查结果提供了关于在药物输送和生物技术中安全使用IL的见解.
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