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采集玉米 (Zea mays) 叶子微生物群的样本

Jason G Wallace^1,2,3

¹Department of Crop and Soil Science, University of Georgia, Athens, Georgia 30602, USA jason.wallace@uga.edu.

Cold Spring Harbor protocols

|September 5, 2024

概括

本研究详细介绍了采样玉米叶微生物群的方法,区分了表面表皮和内部内皮. 这些协议为微生物分离或DNA测序准备了叶子组织,有助于研究植物微生物相互作用.

科学领域:

植物病理学植物病理学
微生物学微生物学
农业科学农业科学

背景情况:

玉米叶微生物组包括可以影响植物健康的微生物,具有有益和有害的影响.
虽然叶子疾病显然会产生负面影响,但典型的叶子微生物群体的微妙作用正在积极调查中.

研究的目的:

提出用于有效采样玉米叶微生物群的标准化方案.
区分玉米叶的表层微生物群落和内层微生物群落.

主要方法:

该议定书概述了玉米叶组织的两个不同的采样策略.
方法1侧重于收集表面 (表层) 微生物群.
方法2的目标是内部 (内细胞) 微生物群.

主要成果:

这两种方法都从收集的叶子组织中产生细胞悬浮物.
由此产生的悬浮物适用于下游应用,例如活微生物隔离.
作为另一种选择,该悬浮液可以用于用于微生物社区测序的DNA提取.

结论:

标准化协议对于可复制的玉米叶微生物组研究至关重要.
这些方法有助于研究与玉米叶相关的致病性和开始性微生物.
了解叶子微生物群是管理植物健康和农业生产率的关键.

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